ISLH Recommended Reference Procedure for the Enumeration of Particles in Urine
T. KOURI1, A. GYORY2, R. M. ROWAN3
1Oulu University Hospital, Oulu, Finland; 2Royal North Shore Hospital, St. Leonard?s, Australia; 3Glasgow, Scotland
Members of ISLH Urinalysis Task Force:
T. DEMATTEO, USA; M. DOTSON, USA; B. FERNANDES, CANADA; A. GYORY, AUSTRALIA;
A. KOONTZ, USA; T. KOURI, FINLAND; A. MICHAELS, USA; M. ROWAN, SCOTLAND
Adviser to the Task Force:
PROFESSOR P. LAIPPALA, PHD, TAMPERE SCHOOL OF PUBLIC HEALTH, TAMPERE UNIVERSITY;
RESEARCH UNIT OF TAMPERE UNIVERSITY HOSPITAL, TAMPERE, FINLAND
FOREWORD
Automated systems for counting urine particles are now
being developed and marketed. A reference measurement
procedure must be developed to determine the accuracy and
validity of these systems and to provide means for instrument
calibration.
A task force of the International Society of Laboratory
Hematology (ISLH) has proposed such a procedure for the
enumeration of erythrocytes, leukocytes, hyaline casts, and
squamous epithelial cells in urine. The proposed standard
can be extended to include other urine particles such as the
smaller epithelial cells and granular or cellular casts when
there is consensus on the morphological description of
these entities.
The ISLH Task Force standard is based on ISO/DIS (International
Organization for Standardization) draft international
standard 78-2, with special consideration given to the requirements
for biological materials and for reference measurement
procedures, the current model being based on the structures
being developed by CEN/TC (European Standardisation
Committee, Technical Committee on Health Care Informatics)
140 prEN 12286 and ISO/CD (ISO committee draft)
15193, with reference to ISO/CD 15195. Where relevant the
terms and definitions are those provided in the International
Vocabulary of Basic and General Terms in Metrology (VIM) [1].
The proposed standard draws heavily on previously published
guidelines such as the Finnish Recommendations from
the Working Group on Clean Midstream Specimens [2], the
NCCLS Urinalysis and Collection, Transportation and Preservation
of Urine Specimens (NCCLS Document GP16-A) [3],
JCCLS (Japanese Committee for Clinical Laboratory Standards
Guideline) GP1-P2 [4], and the ECLM (European
Confederation of Laboratory Medicine) European Urinalysis
Guidelines [5].
The importance of preanalytical specimen information is
usually underestimated, and this information is generally not
documented. This information is relevant and must be
included in the procedure.
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