Capillary Electrophoresis for Characterization of Low Molecular Weight Heparins
I. RAMASAMY, J. KENNEDY, K. TAN
Therapeutic Goods Administration, Canberra, Australia
ABSTRACT:
In many instances, low molecular weight heparins
(LMWH) have replaced unfractionated heparins for prevention
and treatment of venous thromboembolism. Each
LMWH is a specific compound with its own physicochemical
and pharmacological properties. These properties
are critical in determining the therapeutic efficacy of
the product. In recent times, capillary electrophoresis
(CE) has emerged as a means of analytical separation of
biological molecules. There are few reports, however,
detailing the separation of whole heparins by capillary
electrophoresis. This paper reports the use of CE to characterize
LMWH without prior oligosaccharide release,
labeling, or derivatization. The paper also focuses on the
advantages of CE separation for the monitoring of product
consistency.
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